Engine Protein Arrays

Pioneers with more than 15 years of experience

Screen 10.000 antigens in a hypothesis-free protein array analysis.

Our protein array service increases your chance of achieving new and potentially decisive discoveries. Our protein arrays present over 10.000 human proteins and peptides derived from different tissues. 

The corresponding cDNA expression libraries were validated, and advanced in more than 15 years of experience.

We offer different types of protein arrays and also custom protein arrays, which can be generated from subsets of our libraries. Size range from 500 up to 13.000 clones spotted in duplicates.

Our method is published in over 100 studies to date.

engine protein array mit more than 10000 antigens
Explore the protein universe

How to manufacture a protein array?

Watch our video of our robot „Baloo“. (2:00 min)

1. The roboter arm picking up a microtiter plate, containing 384 individual E. coli clones of a cDNA expression library from the hotel.

2. The plate cover is lifted off automatically.

3. The gadget, consisting of 384 steel needles, carefully stirs the E. coli clones.

4. The clones are spotted onto the PVDF membrane.

5. After spotting, the PVDF membranes are placed on agar plates and E. coli clones are grown over night and protein expression is induced.

Our Protein Arrays

We offer different types of protein arrays wich present over 10.000 human proteins and peptides derived from different tissues based on a human fetal brain, T-cells, lung and colon cDNA libaries. Our engine protein arrays based on the technique of Source Biosciences, imaGenes and RZPD.

hEXselect Protein Array

Product no. 1003

Human fetal brain cDNA librarie.

In total, >23.000 clones represent
>6.000 distinct human proteins and peptides.

Size: 22×22 cm (8.7×8.7 inch)

UniPEx Protein Array

Product no. 1008

Human fetal brain, T-cells, lung and colon cDNA librarie

In total, >15.000 clones represent
>7.000 distinct human proteins and peptides. 

Size: 22×22 cm (8.7×8.7 inch)

Custom Protein Array

We offer custom protein arrays, which can be generated from subsets of our human fetal brain, T-cells, lung and colon cDNA libraries. Size range from 500 up to 13.000 clones spotted in duplicates. 

Investigate disease-related protein modifications

Our protein arrays can be modified enzymatically to investigate disease-related protein modifications, if citrullination, phosphorylation or carbamylation are disease-associated or causative. For example, antibodies against cyclic citrullinated peptides in rheumatoid arthritis.

Citrullination

Phosphorylation

Carbamylation

Testimonials

Don’t take our word for it– here’s what our customer say about biomarker discovery with engine protein arrays:

We used the protein macroarrays to screen for protein-protein interaction (PPI) partners using fluorophore-labelled peptide baits. This enabled us to identify the histone lysine Nmethyltransferase EHMT2/G9a as an interaction partner and functional modulator of trans-activation domains of transcription factors of the C/EBP family (1).

Ole Pless;
Fraunhofer IME Screening Port, Hamburg, Germany

We used the hEXselect multiprotein-array to identify interaction partners of antisera directed to bacteria, which are suspected to cause schizophrenia in the offspring of mothers infected during early pregnancy. (1,2) By this we could identify a handful of interesting candidate molecules which have later been functionally characterized.

Bernhard Reuss;
University Medical Center Göttingen

To our experience, the protein arrays are super-informative and yield highly reproducible results specifically for biomarker discovery and validation. We have screened Testis, Fetal hEX1, UniPEx and hEXselect protein arrays to identify autologous antibodies specific to Small Cell Lung Cancer (SCLC), Non-small Cell Lung Cancer (NSLCL), Ovarian Cancer, Colorectal Cancer, Gastric Cancer, Behcet’s disease and Neuromyelitis optica (NMO) in comparison with healthy controls.

Şükrü Atakan;
Bilkent University, Turkey