Content of engine Protein Arrays
Explore our protein universe and discover novel
biomarker
Our hEXselect and UniPEx protein arrays consist of more than 10,000 human antigens. They cover a broad range of the human proteome and give you the opportunity to analyze interactions with
full-length proteins, isoforms, peptide and also neoantigens such as frameshift peptides as potential biomarkers.
Our antigens include major functional classes, covers different tissues and are involved in a whole range of diseases.
Content of hEXselect Array
The hEXselect Array (product no. 1003) has > 57,000 spots and > 19,000 human antigens which represents > 4,000 different human genes.
Content of UniPEx Array
The UniPEx Array (product no. 1008) has > 36,000 spots and > 14,000 human antigens which represents > 5,000 different human genes.
Controls and human protein & peptide expressing E. coli clones are spotted onto a PVDF membrane. Guiding spots, as well as blanks and E. coli clones with empty vectors are used as controls. The E. coli clones are spotted at least in duplicate. An interaction (e.g. serum antibody binds to antigen) is considered positive (hit) if the signal-intensities of both clones of the duplicate are clearly higher than the signal from the blank and empty vector controls. In this way, we can ensure that no non-specific bindings are evaluated as false positives.
We differentiate our human antigens on the arrays into full-length proteins, protein isoforms & peptides and neoantigens & frameshift peptides. Genes can be represented as full-length protein, isoform and peptide. Frameshift mutations are commonly the cause of severe pathologies so neoantigens and frameshift peptides are relevant in different diseases. Take a look at our newsblog articel for Frameshift Mutation in Cancer Research.
Our combination of established, published & well-characterised antigens and neoantigens enables you to discover hypothesis-free & unbiased novel biomarkers and increases your chance for new, decisive discoveries!
Our arrays cover a broad range of the human proteome
Our antigen library consists of >27,000 different human antigens, represented at hEXselect and / or UniPEx Array. To give you an insight on how these antigens cover the human proteome, we analyzed our > 1,500 different full-length proteins with the Human Protein Atlas and display in the following charts the general protein classes, tissue expression and disease involvement. Please note that proteins can also be part of several groups, like expression in several tissues.
General Protein Classes
According to the protein class division of the Protein Atlas
https://www.proteinatlas.org/humanproteome/proteinclasses the chart gives you an insight on protein subclasses of our full-length proteins.
Tissue Expression
Our human protein expression library derived from human fetal brain, CD4+ T-cells, lung and colon cDNA, but our antigens cover a broader range of
tissue expression, like you can see in the chart. The data is based on the tissue specific RNA details of the Protein Atlas
https://www.proteinatlas.org/humanproteome/tissue.
Disease Involvement
Via an analysis using the Protein Atlas, we were able to identify that of our full length proteins are involved in 88 different diseases. The chart gives an insight into the different diseases. With novel biomarker discovery this list
will probably expand!
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