hEXselect
human protein array

The hEXselect protein array contains > 57, 000 spots with > 19,000 distinct human antigens. The human protein expression library includes recombinantly expressed proteins, derived from human fetal brain cDNA. All clones have been 5’tag sequenced and are fully annotated. It comprises full-length as well as shorter cDNA clones. The expressed human proteins are present as a mixture of partially de- and renaturated conformation.

The original protein expression library was evaluated by in silico analysis and genes with a high clone number were reduced to decrease redundancy. For genes represented by a single clone, the clone was doubled to increase recovery rate. The size of the low redundancy collection allows a complete spotting in duplicates onto a single human protein array, saving processing time and hybridization probe volume, compared to the original protein expression library. Most of the E. coli clones represent partial proteins, which may include artificial protein sequences corresponding to 5’ and 3’UTR sequences.

Find out more about protein classes, tissue expression & disease involvement in regard to our array content

Protein Array Service

We support you in biomarker discovery and the determination of antibody specificity. With our protein array analysis we offer full service around protein arrays.

Product No.:

1003

Source:

Human fetal brain cDNA

Expression host:

E. coli

Number of spots:

57,600 spots in total,
9,178 (15.9 %) controls,
48,422 protein-coding E. coli clones

Number of distinct antigens/genes:

19,453 different antigens which represent > 4,000 different genes

Controls & multiple determination:

Controls are guiding spots, blanks & E. coli clones with empty vectors.
Protein-coding E. coli clones are spotted at least in duplicate.

Protein conformation:

Mixture of partially de-and renaturated

Protein length & ORF:

3,107 different in-frame full-length proteins (12.3 % of spots in total); 7,539 in-frame protein isoform variants & peptides (29.9 % of spots in total); 10,537 neoantigens & frameshift peptides (41.8 % of spots in total)

Tag (for detection and purification purposes):

N-terminal RGS-His6

Array matrix and size:

PVDF-membrane, 222mm x 222mm (approx. 8.7 inch x 8.7 inch)

Price:

1.100 € per array, bulk discount possible

Testimonials of hEXselect Protein Arrays

Take a look to our costumer voices about their experience with our human protein arrays.
You have already worked with our arrays? Send us your experience report!

We used the hEXselect multiprotein-array to identify interaction partners of antisera directed to bacteria, which are suspected to cause schizophrenia in the offspring of mothers infected during early pregnancy. (1, 2) By this we could identify a handful of interesting candidate molecules which have later been functionally characterized. In the course of these experiments, the hEXselect multiprotein-array turned out to be a highly valuable tool that is comparably easy to handle, making the experimental procedures nearly as simple as a western blot! However, even more important for our project was the fact, that the cDNAs in the expression library used for this array, were derived from the frontal cortex of two human fetal brains of the late first and the early second trimester of pregnancy, thereby covering in terms of schizophrenia pathology almost perfectly the expression pattern during the most vulnerable phase of prenatal brain development.

1 Almamy A, Schwerk C, Schroten H, Ishikawa H, Asif AR, Reuss B. 2017. Crossreactivity of an Antiserum Directed to the Gram-Negative Bacterium Neisseria gonorrhoeae with the SNARE-Complex Protein Snap23 Correlates to Impaired Exocytosis in SH-SY5Y Cells. J Mol Neurosci 62(2):163-180. DOI: 10.1007/s12031-017-0920-2

2 Almamy A, Schwerk C, Schroten H, Ishikawa H, Asif AR, Reuss B. 2017. Interactions of antisera to different Chlamydia and Chlamydophila species with the ribosomal protein RPS27a correlate with impaired protein synthesis in a human choroid plexus papilloma cell line. Immunol Res 65:1110-1123. DOI: 10.1007/s12026-017-8952-9

hexselect engine protein array
Bernhard Reuss, PhD

Professor for Cellular Neuroanatomy,
University Medical Center Göttingen

To our experience, the protein arrays are super-informative and yield highly reproducible results specifically for biomarker discovery and validation. We have screened Testis, Fetal hEX1, UniPEx and hEXselect protein arrays to identify autologous antibodies specific to Small Cell Lung Cancer (SCLC), Non-small Cell Lung Cancer (NSLCL), Ovarian Cancer, Colorectal Cancer, Gastric Cancer, Behcet’s disease and Neuromyelitis optica (NMO) in comparison with healthy controls. We were able to identify disease specific autologous antibodies and validate them via custom-made arrays. The nature and format of the protein arrays provided us the opportunity to develop a screening protocol by which we were able to generate signals in a broad dynamic range, obtain high signal/background and positive signal/negative signal ratios. Further, we could identify novel autologous antibodies which we will publish after validation with ELISA and Western blot.

Sukru Atakan used hexselect and unipex engine protein array
Şükrü Atakan, Postdoctoral Researcher

Bilkent University, Turkey

More Inspiration?

Have a look at our publication list with more than 100 studies using engine protein arrays. Our arrays based on the technique of Source Biosciences, imaGenes and RZPD.