Applications & Publications

Protein arrays for your research

Protein arrays open up manifold possibilities as they can be used for analysing protein interactions with numerous types of molecules. You can determine auto-antibody profiles of complex sera, cross-reactivity of monoclonal antibodies, protein-protein interaction pairs, DNA/RNA binding proteins, and much more.

For more than 20 years our protein arrays, also called high-density filters, have been used in biomarker research all over the world and for various indications. Whether you want to discover new biomarkers for diagnostics or therapy, or gain a deeper understanding of disease or metabolic pathways. Get an overview of your research area in our  database of publications!

Research areas

Protein arrays accelerate your research from hypothesis to validation.

Diseases & pathways

  • Apoptosis
  • Autoimmune diseases
  • Cancer
  • Cardiovascular disease
  • Corona virus
  • Diabetes
  • Food, microbiome, lifestyle
  • Infection & vaccination
  • Inflammation
  • Kidney disease
  • Neurobiology
  • Signalling pathways

Target interactions

  • Antibody profiling (e.g. IgM, IgG, IgA)
  • Antibody specificity & off-targets
  • DNA/RNA binding
  • Enzymatic modifications
  • Immune response profiling
  • Protein-protein interactions
  • Small molecule profiling

Biomarker applications

  • Companion diagnostics
  • Diagnostics
  • Drug discovery & therapeutics
  • Monitoring
  • Personalized medicine
  • Risk stratification & prognostic
  • Prediction & response
  • Vaccination

Spotlighted publications

Case studies

Neurobiology

Validation of Plasma Proteomic Biomarkers Relating to Brain Amyloid Burden in the EMIF-Alzheimer’s Disease Multimodal Biomarker Discovery Cohort

Diagnosing Alzheimer’s disease at pre-symptomatic stages is the key to decelerating disease progression, but it is a costly and invasive process as it requires lumbar puncture. By screening patient plasma samples using protein arrays, mass spectrometry, and immunocaputre methods, the group could identify a 7-protein biomarker panel which is predicted to reduce the screen failure rate by 20% .

Westwood, Sarah et al. “Validation of Plasma Proteomic Biomarkers Relating to Brain Amyloid Burden in the EMIF-Alzheimer’s Disease Multimodal Biomarker Discovery Cohort.” Journal of Alzheimer’s disease : JAD vol. 74,1 (2020): 213-225. doi:10.3233/JAD-190434

Corona virus

Gene expression profiling of corona virus microarray datasets to identify crucial targets in COVID-19 patients

In order to gain a better insight into the mechanism behind SARS-CoV immune dysregulation associated with high mortality, large amounts of microarray data were gathered. With the help of this dataset of normal and severe COVID-19 patients, a protein-protein network could be constructed. Hub genes could be identified (e.g CAMP, ELANE, LTF), the dysregulation of which is associated with high mortality rates.

Ramesh, Priyanka et al. “Gene expression profiling of corona virus microarray datasets to identify crucial targets in COVID-19 patients.” Gene reports vol. 22 (2021): 100980. doi:10.1016/j.genrep.2020.100980

Cancer

Combination of Autoantibody Signature with PSA Level Enables a Highly Accurate Blood-Based Differentiation of Prostate Cancer Patients from Patients with Benign Prostatic Hyperplasia

Using Hex1 high-density protein macroarrays, sera of patients with prostate cancer were screened for disease-specific autoantibodies. The applied method can distinguish prostate cancer patients from normal controls with an accuracy of 83.2%, making the identified biomarkers are thus an important diagnostic tool.

Leidinger, Petra et al. “Combination of Autoantibody Signature with PSA Level Enables a Highly Accurate Blood-Based Differentiation of Prostate Cancer Patients from Patients with Benign Prostatic Hyperplasia.” PloS one vol. 10,6 e0128235. 3 Jun. 2015, doi:10.1371/journal.pone.0128235

Signalling pathways

Identification of a high-affinity network of secretagogin-binding proteins involved in vesicle secretion

The cellular interaction network of the protein secretagogin, which is known for its support for hierarchical organizational principles in the mammalian brain, was expanded by nine proteins through protein array screenings. The interactions of the identified proteins were verified by means of a GST pulldown assay. The interacting proteins form a specific network that mediates secretion and vesicle transport. This suggests a regulating role for secretagogin in these processes.

Bauer, Mikael C et al. “Identification of a high-affinity network of secretagogin-binding proteins involved in vesicle secretion.” Molecular bioSystems vol. 7,7 (2011): 2196-204. doi:10.1039/c0mb00349b

Autoimmune disease

Immunogenicity of autoantigens

This paper examined which structural or biological features of certain auto-antigens and tumor-associated antigens trigger an immune response. In-silico methods were used to analyze large structural databases of previously identified antigens, identified with the help of various human protein array screenings. The authors state that proteins that are evolutionarily conserved, have certain sequence motifs or are part of cellular structures are more likely to trigger auto-immunogenic reactions.

Backes, Christina et al. “Immunogenicity of autoantigens.” BMC genomics vol. 12 340. 4 Jul. 2011, doi:10.1186/1471-2164-12-340

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Protein Array Research Database

Science is based on sharing knowledge and building on the efforts of others. With this publication database, we would like to honour the valuable work of scientist – and support you in your research. We will be pleased if this provides you with inspiration, helpful information or even makes you aware of possible collaborations.

What’s inside?

  • Protein array related publications, dealing with specific diseases such as spondyloarthritis
  • Studies on overall indications such as autoimmunity
  • Reviews and publications focussing arrays from the technical, methodological perspective
  • Exciting papers with peptide arrays or other protein arrays

How to use the database

Search by full-text search or categories and keywords to find hits from your desired research field.

We are happy to include new publications! Feel free to contact us, if you have any study for our database. Please let us also know if something is not working :-).

Please note that the table is only shown in desktop version.

Simply enter your search term, e.g.:

  • Autoimmune Diseases
  • Cancer
  • Biomarker Discovery
  • Signalling Pathways
  • Methods/Technology
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