Protein arrays for your research

Protein arrays can be used for analysis of protein interactions with numerous types of molecules. So they can be applied to determine auto-antibody profiles of complex sera, cross-reactivity of monoclonal antibodies, protein-protein interaction pairs, DNA/RNA binding proteins, etc.

Scientists from all over the world have used the protein arrays in their R&D successfully.

Validation of Plasma Proteomic Biomarkers Relating to Brain Amyloid Burden in the EMIF-Alzheimer’s Disease Multimodal Biomarker Discovery Cohort

Diagnosing Alzheimer’s disease at pre-symptomatic stages is the key to decelerating disease progression, but it is a costly and invasive process as it requires lumbar puncture. By screening patient plasma samples using protein arrays, mass spectrometry, and immunocaputre methods, the group could identify a 7-protein biomarker panel which is predicted to reduce the screen failure rate by 20% .

Westwood, Sarah et al. “Validation of Plasma Proteomic Biomarkers Relating to Brain Amyloid Burden in the EMIF-Alzheimer’s Disease Multimodal Biomarker Discovery Cohort.” Journal of Alzheimer’s disease : JAD vol. 74,1 (2020): 213-225. doi:10.3233/JAD-190434

Gene expression profiling of corona virus microarray datasets to identify crucial targets in COVID-19 patients

In order to gain a better insight into the mechanism behind SARS-CoV immune dysregulation associated with high mortality, large amounts of microarray data were gathered. With the help of this dataset of normal and severe COVID-19 patients, a protein-protein network could be constructed. Hub genes could be identified (e.g CAMP, ELANE, LTF), the dysregulation of which is associated with high mortality rates.

Ramesh, Priyanka et al. “Gene expression profiling of corona virus microarray datasets to identify crucial targets in COVID-19 patients.” Gene reports vol. 22 (2021): 100980. doi:10.1016/j.genrep.2020.100980

Combination of Autoantibody Signature with PSA Level Enables a Highly Accurate Blood-Based Differentiation of Prostate Cancer Patients from Patients with Benign Prostatic Hyperplasia

Using Hex1 high-density protein macroarrays, sera of patients with prostate cancer were screened for disease-specific autoantibodies. The applied method can distinguish prostate cancer patients from normal controls with an accuracy of 83.2%, making the identified biomarkers are thus an important diagnostic tool.

Leidinger, Petra et al. “Combination of Autoantibody Signature with PSA Level Enables a Highly Accurate Blood-Based Differentiation of Prostate Cancer Patients from Patients with Benign Prostatic Hyperplasia.” PloS one vol. 10,6 e0128235. 3 Jun. 2015, doi:10.1371/journal.pone.0128235

Identification of a high-affinity network of secretagogin-binding proteins involved in vesicle secretion

The cellular interaction network of the protein secretagogin, which is known for its support for hierarchical organizational principles in the mammalian brain, was expanded by nine proteins through protein array screenings. The interactions of the identified proteins were verified by means of a GST pulldown assay. The interacting proteins form a specific network that mediates secretion and vesicle transport. This suggests a regulating role for secretagogin in these processes.

Bauer, Mikael C et al. “Identification of a high-affinity network of secretagogin-binding proteins involved in vesicle secretion.” Molecular bioSystems vol. 7,7 (2011): 2196-204. doi:10.1039/c0mb00349b

Immunogenicity of autoantigens

This paper examined which structural or biological features of certain auto-antigens and tumor-associated antigens trigger an immune response. In-silico methods were used to analyze large structural databases of previously identified antigens, identified with the help of various human protein array screenings. The authors state that proteins that are evolutionarily conserved, have certain sequence motifs or are part of cellular structures are more likely to trigger auto-immunogenic reactions.

Backes, Christina et al. “Immunogenicity of autoantigens.” BMC genomics vol. 12 340. 4 Jul. 2011, doi:10.1186/1471-2164-12-340

A method for global protein expression and antibody screening on high-density filters of an arrayed cDNA library

This publication presents a technique for global protein expression and antibody screening. A similar fundamental approach is also employed in the Engine Protein Arrays. The authors used a human fetal brain cDNA library, which was then cloned into bacterial vectors. Afterwards, the library was arrayed, protein expression was induced on a PVDF filter and the finished, processed protein array was examined. Monoclonal antibodies against a globally expressed N-terminal tag were employed and furthermore, two example proteins were detected via protein-specific antibodies.

Büssow, K et al. “A method for global protein expression and antibody screening on high-density filters of an arrayed cDNA library.” Nucleic acids research vol. 26,21 (1998): 5007-8. doi:10.1093/nar/26.21.5007

Protein Array Research Database

In 1998 K. Büssow et al. cloned and selected a human fetal brain cDNA library in E. coli (hEx1 library) in a collaboration between the Max Planck Institute (MPI) for Molecular Genetics and the Resource Center of the German Human Genome Project (RZPD). The clones were then spotted as high-density filters and the expressed proteins were detected by antibodies. This work forms the basis for the engine protein arrays. The hex1 arrays were further developed from 2007 onwards by the RZPD spin-off imaGenes GmbH (later Source BioScience) to the hEXselect Array. The cDNA library for the high-density protein arrays was extended by protein expression libraries from human CD4+ T-cells, lung, colon so the UniPEx Array was created.

For more than 20 years these high-density filters have been used in biomarker research all over the world and for various indications.

With this publication database we want to honor the work, the research and the scientists. Science is based on sharing knowledge and building on the efforts of others. Therefore, we have listed protein array related publications, dealing with specific diseases such as spondyloarthritis, papers on the overall indication such as autoimmunity, but also reviews and publications which focused on the technical, methodological side of the arrays. And we have also included exciting papers with peptide arrays or other protein arrays.

We would like to use this little database to help you with your research, and we would be happy if it gives you inspiration, food for thought or even makes you aware of possible collaborations.

Be part of the protein array research community!

The database has a full-text search, and we have added categories and keywords to the publications so that you can search for your research field as efficiently as possible.

Feel free to contact us if you have any publications for our database or if, for example, links are no longer up to date.

Please note that the table is only shown in desktop version.

Simply view the results for e.g. :

  • Autoimmune Diseases
  • Cancer
  • Biomarker Discovery
  • Signalling Pathways
  • Methods/Technology
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