1. Roboter arm pick­ing up a microtiter plate, con­tain­ing 384 indi­vidu­al E. coli clones of a cDNA expres­sion lib­rary from the hotel.
2. Plate cov­er is lif­ted off auto­mat­ic­ally.
3. The gad­get, con­sist­ing of 384 steel needles, care­fully stirs the E. coli clones.
4. Clones are spot­ted onto the PVDF mem­brane.
5. After spot­ting, the PVDF mem­branes are placed on agar plates and E. coli clones are grown over night and pro­tein expres­sion is induced.

How to use our arrays

After the arrays are pro­duced, they can be eas­ily stored at room tem­per­at­ure and shipped world­wide. You need only 50 µl sample, vari­ouse mat­rix is pos­sible, e.g. ser­um, CSF or cell super­natant. The incub­a­tion sheme is as simple as a West­ern Blot.

1. Rehyd­ra­tion of array (PDVF mem­brane)
2. Remov­al of E. coli debris
3. Wash­ing
4. Block­ing
5. Incub­a­tion with sample
6. Wash­ing
7. Incub­a­tion with sec­ond­ary anti­body
8. Detec­tion with sub­strate
9. Soft­ware-based eval­u­ation