Learn about validation of array-based antibody-protein interactions using Luminex bead arrays
Have you ever struggled with confirmation of your previously identified candidate biomarkers? Antibody validation after initial screening can be tedious and time-consuming. Together with our cooperation partner, the Austrian Institute of Technology (AIT) we have conducted a validation experiment and tested sera from rheumatoid arthritis (RA) patients for interaction on engine protein arrays. The results of this experiment were validated by using the complete certified immunomics workflow implemented at AIT for screening, confirmation and validation of autoantibodies.
Learn more about this validation study in our application note.
Learn about the sensitivity of engine protein arrays compared to commercially available ELISA using the example of TRIM21
ELISA are commonly used for diagnostic purposes. Therefore, the question arises whether engine protein arrays have comparable sensitivity and specificity to ELISA and whether the interactions found with engine protein arrays can be reproduced in an ELISA screen and vice versa. Therefore, engine performed a series of experiments to investigate how their hEXselect and UniPEx arrays compare to a commercial ELISA for the antibody effector protein TRIM21 (Tripartite motif-containing protein 21).
Learn more about this study in our application note.
Learn about posttranslational protein modifications on engine protein arrays, using the example of protein phosphorylation with protein kinase A
Posttranslational modifications (PTMs) are enzymatic modifications of proteins following protein biosynthesis in the cell. Many different PTMs are known, and some can be introduced to proteins in vitro.
In this study we focus on cAMP-dependent protein kinase A (PKA) aiming to identify targets and thereby getting some more insight into the target preference of the kinase.
Learn more about phosphorylation of proteins by PKA in this application note.